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ELISA 样品制备指南
浏览次数:91发布日期:2020-08-17

ElisaSHENGWUSHIYANSHIYIZHONGMINGANXINGGAO,TEYIXINGQIANG,ZHONGFUXINGHAODESHIYANZHENDUANFANGFA。YINWEIQISHIJIANWEN、YIBAOCUN,CAOZUOJIANBIAN,CHENGGUOPANBIEJIAOKEGUANDENGYAOSU,YIGUANGFANYINGYONGZAIMIANYIXUECHAYANDEGELINGYUZHONG。ELISAJIANCESHIJIHESHIYONGYUTIWAIDINGXINGJIANCERENXUEQINGHUOXUEJIANGZHONGDEKANGRENLEIWUXINGGANYAN(HEV)BINGDU IgM KANGTIELISAJIANCE。

 

ZAISHIYANZHONG,YANGPINDEZHIBEIYESHIFEICHANGZHONGYAODE,CAOZUOQIANHAOKENENGHUIYINGXIANGSHIYANCHENGGUO,JIUJINGGAIZENMEZHIBEIYANGPINNI?JIEXIALAIXIAOBIANJIUDAINIKANKANELISAYANGPINZHIBEIDEFANGFABA!

 

1.XIBAOPEIYUWUSHANGQINGYE

 

①JIANGPEIYUJIYIZHILIXINGUAN, 4℃, 1,500 rpm LIXIN 10 FENZHONG;

②DANGJIJINXINGSHANGQINGYEDEFENZHUANG(XUEQING)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

2.XIBAOTIQUWU

 

零花钱①JIANGANPAIPEIYUBANFANGZHIZAIBINGSHANG;

②XICHUPEIYUJIBINGYONGYULENGPBS QINGQINGXIDIXIBAOYICI;

③XICHU PBS ,CANJIA100 mm PEIYUBANCANJIA0.5mlCHEDITIQUHUANCHONGYE;

④SOUJIXIBAOZHIZAIWAIXIEBANZHONG,RANHOUYIZHITONGGUOYULENGDELIXINGUANZHONG;

⑤DUANZANWOXUANHOUZAIBINGSHANGFUYU 15-30 FENZHONG;

⑥ZAI 4℃ XIA13,000 x rpm LIXIN 10 FENZHONG,CHENJIBURONGXINGNEIRONGWU;

⑦JIANGSHANGQINGYE(ZHEILISHIZHIKERONGXINGXIBAOTIQUWU)FENZHUANGZHIYULENGDELIXINGUANZHONG,BINGYU -80℃ BAOCUN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

3.TIAOJIANPEIYUJI

 

零花钱①JIANGXIBAOJIEZHONGDAOCHEDI(HANXUEQING)CHENGZHANGPEIYUJIZHONG,SHIXIBAOZENGZHIDADAOYIDINGDEJIAORONGLV;

零花钱②QIQUPEIYUJI,SHUHAOSHENGYUREPBS DANGXINXIDI,ZHONGFUXIDIBUZHOU;

③QIQUPBS XIDIYEBINGDANGXINCANJIAWUXUEQINGPEIYUJI;

④FUYU 1-2 TIAN;

⑤JIANGPEIYUJIYIZHILIXINGUAN, 4℃ 1,500 rpm LIXIN 10 FENZHONG;

⑥DANGJIFENZHUANGSHANGQINGYE(XUEQING)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

4.RUZHI

 

①SOUJIYANGPINBINGZAI 4℃ XIAYI 10,000 x g LIXIN 2 FENZHONG;

②FENZHUANGSHANGQINGYEBINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

5.XUEJIANG

 

①JIANGQUANXUESOUJIDAOHANKANGNINGJIDEGUANZHONG,LIRU BD KANGNINGZHENKONGCAIXUEGUANHUOZENGJIA 0.1M NINGMENGSUANNAZHI 1/10 ZHONGTIJI;

②4℃ 3,000 rpm LIXIN 10 FENZHONG;

③DANGJIFENZHUANGSHANGQINGYE(XUEJIANG)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

6.NIAOYE

 

零花钱①SOUJIYANGPINBINGZAI 4℃ XIAYI 10,000 x g LIXIN 2 FENZHONG;

②DENGFENSHANGQINGYE(XUEQING)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANDONGRONGXUNHUANCISHU。

 

7.TUOYE

 

①SOUJIYANGPINBINGZAI 4℃ XIAYI 10,000 x g LIXIN 2 FENZHONG;

②DANGJIFENZHUANGSHANGQINGYE(XUEJIANG)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

8.XUEQING

 

零花钱①JIANGQUANXUESOUJIDAOWEIJINGCHULIDECESHIGUANZHONG,HUOXUDAOBUHANKANGNINGJIDEGUANZHONG,RU BD XUEQINGYONGZHENKONGCAIXUEGUAN;

②ZAISHIWENXIALIANXUFUYU 20 FENZHONG;

零花钱③4℃ 3,000 rpm LIXIN 10 FENZHONG;

④DANGJIFENZHUANGSHANGQINGYE(XUEJIANG)BINGZAI -80℃ XIABAOCUNYANGPIN,JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

9.ANPAITIQUWU

 

①YONGJIEJINGDEDONGXIJIEPOUANPAI,bestHAOZAIBINGSHANG、GANKUAIJINXING,YIBIMIANBEIDANBAIMEIJIANGJIE;

②JIANGANPAIZHIYUYUANDIDEWEILIANGLIXINGUANZHONG,BINGJINRUYEDANZHONGJINXING“SUDONG”,JIANGYANGPINBAOCUNZAI -80℃ XIAYIBIANHOUXUYUNYONGHUOFANGZHIZAIBINGSHANGJINXINGZHIJIEYUNJIANG;

③DUIYUYUE 5 mg DEANPAIPIAN,KEXIANGGUANZHONGZENGJIAYUE 300 μl CHEDITIQUHUANCHONGYE(JIANCHAXIBAO/ANPAITIQUHUANCHONGYEPEIFANG),RANHOUYONGDIANDONGYUNJIANGQIYUNJIANG;

④QINGXIDAOPIANLIANGCI,MEICIQINGXIYUNYONG 300μl CHEDITIQUHUANCHONGYE,RANHOUZAI 4℃ XIAWEICHIHENGDINGBANHE 2 XIAOSHI(LIRUZHIYULENGSHIZHONGDEHUIXUANZHENDANGQISHANG);

⑤4℃ 13,000 x rpm LIXIN 20 FENZHONG。ZHIYUBINGSHANG,JIANGSHANGQINGYE(ZHEILISHIZHIKERONGDEDANBAIZHITIQUWU)FENZHUANGZHIXIN、YULENGDEGUANZHONGBINGZAI -80℃ XIABAOCUNYANGPIN。JINKENENGXUEJIANZHONGFUDONGRONGCISHU。

 

 

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