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您现在的位置:零花钱 >> 技术文章 >> 原代细胞和传代细胞培育4个不同之处
原代细胞和传代细胞培育4个不同之处
浏览次数:109发布日期:2020-08-10

零花钱YUANDAIXIBAOHECHUANDAIXIBAOPEIYUYOUYIYI、PEIYUJINCHENG、PEIYUCHENGGUOHESHIYONGSIGEFANGMIANzhiCHAYI:

1、YIYIBUTONG

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零花钱CHUANDAIPEIYUSHIZHIXUYAOJIANGPEIYUWUFENGECHENGXIAODEBUFEN,CONGTOUJIEZHONGDAOLINGWAIDEPEIYUQIMIN(PING)NEI,ZAIJINXINGPEIYUDEJINCHENG。

2、PEIYUJINCHENGBUTONG

零花钱YUANDAIPEIYUDEJIBENJINCHENGBAOKUOQUCAI、PEIYUCAILIAODEZHIBEI、JIEZHONG、JIAPEIYUYE、ZHIPEIYUTIAOJIANXIAPEIYUDENGBUZHOU,ZAISUOYOUDECAOZUOJINCHENGZHONG,DOUBIXUJIANCHIPEIYUWUJICHENGZHANGHUANJINGDEWUJUN。

传代培育时要注意无菌操作并防止细胞之间的穿插污染。所有操作要尽量接近酒精灯火焰。每次只进行一种细胞的操作。取出长成单层的原代培育细胞,倒掉瓶内培育液,参加消化液。细胞变圆,相互之间不再连片时将消化液倒掉,参加新鲜培育液,吹打,制成细胞悬液。
3、培育成果不同

零花钱YUANDAIPEIYUXIBAOHUIGELIE。YUANDAIPEIYUDEXIBAOYIBANCHUANZHI10DAIZUOYOUJIUBUJIANDANCHUANXIAQULE,XIBAODECHENGZHANGJIUHUICHUXIANZUZHI,DABUFENXIBAOSHUAILAO。XIBAOJIEZHONGHOUYIBANJIXIAOSHINEIJIUNENGTIEBI,BINGKAIDUANCHENGZHANG,RUJIEZHONGDEXIBAOMIDUSHIHE,5TIANDAOYIZHOUJIKEGOUCHENGDANCENG。

CHUANDAIPEIYUYIBANCHUANDAO40DAO50DAI。YIBANZHUANGKUANG,CHUANDAIHOUDEXIBAOZAI2XIAOSHIZUOYOUJIUNENGFUZHEZAIPEIYUPINGBISHANG,2DAO4TIANJIUKEZAIPINGNEIGOUCHENGDANCENG,XUYAOZAICIJINXINGCHUANDAI。

4、SHIYONGBUTONG

YUANDAIXIBAOPEIYUWEIYANTAOSHENGWUTIXIBAODECHENGZHANG、DAIXIE、FANZHITIGONGYOULIDESHOUFA,TONGSHIYEWEIJINHOUCHUANDAIPEIYUCHUANGZAOTIAOJIAN。SHIYONGCIBANFAHAIKEZHIJIEFUWUYULINCHUANGSHIJIAN。LIRU,YONGCONGSHOUSHUZHONGQIECHUDEZHONGLIUXIBAOJINXINGYUANDAIPEIYU,RANHOUYONGGAIPEIYUXIBAOJINXINGKANGAIYAOWUDETIAOXUAN,LAIXIEZHUXUANZEyouYONGDEHUALIAOFANGAN。

零花钱CHUANDAIPEIYUZHUYAOSHIYONGZAIYIXUEFANCHOU,RUKOUQIANGYIXUEFANCHOUCONGTOUGOUJIANJIEGOUZALUANDEYAGEN,HAIYOUGAIJINYANGSHUIXIBAOPEIYUJINENG,KEDUOCIQUDEYOUYONGDIXIBAOKELONG,DADATIGAOPEIYUCHENGGONGLV,ZENGJIALEKEFENXIHEXINGSHULIANG,TIGAOLECHANQIANZHENDUANZUOYEDEZHILIANGHEXIAOYI。

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